100090: Method to Produce Highly Digestible, Pretreated Lignocellulosic Biomass Using Anhydrous Liquid Ammonia


In the continuing push to develop alternative fuels, bioethanol is clearly a viable option. However, if it is to become a truly economical alternative, a more effective and efficient method of processing lignocellulosic biomass must be developed.


Michigan State University has developed a novel lignocellulosic biomass pretreatment process that results in the formation of highly digestible cellulose III allomorph. The method also provides the option to simultaneously extract biologically inhibitory cell-wall extracts such as lignin, lignin decomposition products, xylo-oligosaccharides, and amides using essentially anhydrous liquid ammonia pretreatment (ALAP). This is important because cellulose crystallinity, lignin-carbohydrate complex (LCC) ester linkages, and non-specific enzymes binding to cell wall components (like lignin) are the major rate-limiting steps to efficient cell wall deconstruction. Non-crystalline cellulose (known as amorphous cellulose) has a four-to-five-times higher rate of enzymatic hydrolysis than native crystalline cellulose.



* More readily digestible product: The ALAP process produces amorphous cellulose, which is considered a more highly digestible form of cellulose than native cellulose I.

* Flexibility: Two streams are created by the process: one rich in cellulose and the other in hemicellulose-lignin. Each stream has the potential to be used to produce different products.



* Cellulosic biofuels: The invention would be beneficial to companies focusing on novel lignocellulosic biofuel production processes.

* Biomaterials: The resultant lignin fraction has potential to be used for the production of various biomaterials.


IP Patent Status

Patent pending




Patent Information:


For Information, Contact:

Thomas Herlache
Assistant Director
Michigan State University - Test